The Comparision of Mouse Uterus Alkaline Phosphatase Activity at the Preimplantation Time After Ovarian Hyperstimulation and Progesterone Injection

Purpose: -Nkaline phosphatase(ALP)is a major sialoglycoprotein cell surface and intracellularof some tissues such as reproductive system. The recent investigations showed that the activity of this enzyme altered during the pre implantation and implantaion time and this alteration may correlate with endometrial receptivity. The aim of this study is to determine ALP activity of mouse uterus after ovarian stimulation and progesterone injection on the preimplantation time. Materials and Methods: For this purpose female NMRI mice with age of 6-10 weeks were selected and superovulated using hMG and hCG injection with interval of 48 hours. In one group of hyperstimulated mice daily injection of progesterone subcutaneously were performed. All the experimental and , control groups were pseudopregnanted at the evening of hCG injection. The mice were sacrificed by cervical dislocation and samples were obtained from 1/3 of medial part of uterine horn on 3/5 days after hCG injection. The cryosections were provided with 5 microne thickness. The ALP activity was determined by azo-coupling technique using Alphanaphtole phosphate as substrate. Results: Our results showed that in control and hyperstimulated groups the ALP reaction was observed on the apical and basal epithlium border and their sytoplasm had granular reaction. The ALP reaction of stromal cells was weak whereas, the myometrial reaction was strong in both groups. In hyperstimulated-progestrone injected group this reaction decreased. Conclusion: Thus ovarian hyperstimulation followed with progesterone injection alter the ALP activity during pre-implantation period, which could affect the endometrial receptivity for embryo implantation. It needs more investigation with complementary technique.